The SR-LAB startle response system is the world’s most widely used startle reflex behavioral research system and the most successful for measuring startle habituation, Prepulse Inhibition (PPI) and Fear Potentiated Startle (FPS).
Execute any and all startle reflex testing paradigms using the SR-LAB Software and specialized additional kits—and ensure accuracy using SR-LAB’s proprietary accelerometer which accommodates the rapid force changes in startle testing.
The only system of its kind that expands to 16 test stations, your SR-LAB™ will test a large number of animals at any given time. With SDI’s unique tubular enclosures which eliminate restraint stress and keep the animal in place to gather the most reliable data.
Easily Test the Most Prevalent Test Paradigms
The basic SR-LAB System will test the Startle Habituation and Pre-Pulse Inhibition paradigms with no additional kits required. To test the Fear Potentiated Startle paradigm, simply add on the SR-LAB Potentiated Startle Kit which includes the SDI Shocker (a proprietary design that keeps the animal from determining any patterns), a shock grid, and a cue light. No additional software is required to support the Fear Potentiated Startle paradigm .
One Software Program for All Paradigms
With the SR-LAB software, you can develop and execute any startle reflex testing paradigm. Other systems utilize different software for each paradigm which has two drawbacks: One—you will spend more money, and two—you have no flexibility in designing your own paradigms, and instead are limited to the paradigms offered by the manufacturer.
Be assured all trials are executed without missing any trials using SR-LAB’s concept of a Session containing Trials (discrete tests). Each session will run the animals through all the trials, and save all trial data for later analysis.
Other highlights of SR-LAB™’s software include:
System components include:
ABS Isolation Cabinet – 15” (W) x 14” (D) x 18” (H)
Control Box – 10.25” (W) x 10.25” (D) x 4 3/8” (H)
Small Animal Enclosure – 3 1⁄2” (L) x 1.1” (ID) Up to ~25g
Small/Medium Animal Enclosure – 5” (L) x 1 1⁄2” (ID) Up to ~50g
Medium Animal Enclosure – 6” (L) x 2 1⁄4” (ID) Up to ~150g
Large Animal Enclosure – 8” (L) x 3 1⁄2” (ID) Up to ~450g
X-Large Animal Enclosure- 10″ (L) x 5″ (ID) Up to ~800g
Small/Medium Slotted Animal Enclosure – 5” (L) x 1 1⁄2” (ID)
Medium Slotted Animal Enclosure – 6” (L) x 2 1⁄4” (ID)
Large Slotted Animal Enclosure- 8” (L) x 3 1⁄2” (ID)
X-Large Slotted Animal Enclosure- 10″ (L) x 5″ (ID)
24 lbs. (Isolation Cabinet), 3 lbs. (Control Box)
Isolation Cabinet: ABS plastic, Control Box: Plastic, Animal Enclosure: Acrylic
16 stations per computer
Standard Cable Lengths
Foot shocks, air puffs, PPI LED Light, High Intensity Light, and user-defined stimuli
If the paradigms you need are not included with SR-LAB’s basic kit, add-on kits are available, requiring no additional software or constraints. Add-on kits offered include:
Potentiated Startle Kit
This kit, used for Fear Potentiated Startle testing, includes a shocker with 8 scrambled outputs, shock grid and cue light. The scrambled outputs prevent the animal from discerning a shock pattern assuring excellent results.
Pure Tone Kit
The pure tone kit allows the user a full range of frequencies driven by an external function generator and audio amplifier to deliver tones. You control the frequency and duration of sounds via the SR-LAB software. This approach eliminates any sound clicks present in other systems. The external isodynamic tweeter speaker that is mounted in the top of the SR-LAB cabinet can handle up to 50 KHz. The tone is generated by applying an analog voltage level to the function generator which outputs the corresponding sine wave frequency to the audio amplifier for output. This kit is extremely useful for gap detection startle reflex testing.
The Tactile Kit provides an air puff stimulus, which is generally used as a kinder, gentler alternative to shock.
High Intensity Light Kit
The kit supplies an immense amount of bright light within the test station, which brings on anxiety in the animal. The LED lights give off no heat, which can affect the animal. This kit is used for anxiety potentiated startle testing.
Standardization Unit Sets a Standard Baseline Response
The baseline response of each of your test stations will be set to the same level prior to testing using the Standardization Unit, which attaches to the top of the animal enclosure and places a repeating fixed force on the sensor. Adjust the response level to the guideline numbers in the user’s manual by turning the Response Adjust dial on the side of the test station. After setting one test station, all you need to do is move the Standardization Unit to the next station and repeat. This assures that results data from multiple tests stations can be compared and your resulting analysis is reliable.
A: Because, as with any system, there is small amount of electrical and mechanical noise in the system. Empty chamber values should be small (<10 mV). This noise is accounted for in the system calibration so the net test results aren’t affected by the ambient noise in the chamber. Contact SDI if these values are large. ALSO SEE NEXT FAQ.
A: If the variability is among the chambers when you standardize, make sure that your chambers are on a stable, flat surface - seismic isolation of chambers is important. If a rigid counter cannot be used, consider mounting chambers on concrete blocks. If the variability is low in standardization, but is seen in animal data then there may be a sound problem. See the manual for calibrating sound.
A: Check that all cable connections are correct. If the display is blank the main fuse has failed. If the display shows 0.0 and will not move off this reading the high voltage fuse has failed. A user can remove the top of the shocker to replace either fuse. Extra fuses are stored inside the shocker.
A: Make sure the power supply to the test station is turned on. Check by turning on the light. Check that the response BNC cable going from the test station to the front of the control box is connected securely.
A: Your mouse may be of a strain that loses hearing as they age. Check to make sure they can hear. Many researchers find that mice show a better startle response to tactile rather than acoustic startle stimuli.
A: Sixteen stations.
A: Refer to Geyer, M.A. and Swerdlow, N. R Measurement of Startle Response, Prepulse Inhibition, and Habituation in Current Protocols in Neuroscience (1998) 8.7.1-8.7.15 .
A: The Standardization Unit provides a constant input to the SR-LAB software. This allows each chamber to be adjusted to the same baseline response allowing comparison of test results between test stations.. The Standardization Unit also establishes that test stations are reliable.
A: First, construct your Trials using the commands from the software menu. Then, construct a Session Definition to control the Background level, the Recording granularity, the Inter-Trial Intervals and the number of repetitions. Within the Session Definition, insert the Trial names you want to run in the order you want them run. We strongly recommend you refer to Geyer, M.A. and Swerdlow, N. R Measurement of Startle Response, Prepulse Inhibition, and Habituation in Current Protocols in Neuroscience (1998) 8.7.1-8.7.15 for rat testing and Geyer, M. A. and Stephanie C. Dulawa (2003) Assessment of Murine Startle Reactivity, Prepulse Inhibition, and Habituation, Current Protocols in Neuroscience, 8.17.1 - 8.17.15 for mice testing.